2021-02-23

[public] 459K views, 36.1K likes, 268 dislikes audio only

The Thought Emporium

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BL21 E. coli are by far the most obnoxious bacteria genetic engineers usually work with for 1 simple reason. They are maddeningly difficult to modify and load with new DNA. This wouldn't be an issue if they weren't also one of the best types for producing proteins en mass, and as such have application in everything from pharmaceutical production, to producing ingredients for foods. But after a few years of tinkering with it I finally found a protocol that reliably fixes this irritating issue, and makes it easy to transform them reliably.

Written protocol - https://github.com/thethoughtemporium/Protocols/blob/main/Transformations/bl21%20transformation.md

Sebastian's spinner - https://www.tinkercad.com/things/5t5GqlGkK1i

Previous videos;

Modifying Bacteria - /youtube/video/F0_q-fD_lyU

Modifying yeast - /youtube/video/DHNPnO5UOYQ

Making a heat block/incubator - /youtube/video/Zm378x6zJYc

Growing spider silk -/youtube/video/2hf9yN-oBV4

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