video thumbnail 21:48
Genetically Modifying Yeast, Then Baking With Them! - Beta Carotene Yeast

2020-06-29

[public] 353K views, 28.2K likes, 316 dislikes audio only

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Genetic Engineering is, amazing. In many previous videos we've explored the basics of how genetic engineering works, and today we'll be exploring something a little more complicated; engineering yeast to produce beta-carotene, the precursor for vitamin A. We'll then use these newly modified yeast to bake and brew with, giving the items we make a vitamin boost!

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Igem Team: http://2011.igem.org/Team:Johns_Hopkins

Original Paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932764/

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PLATE solution recipe:

40% PEG3350 (w/v)

100 mM lithium acetate (LiAc)

10 mM Tris, pH 7.5

0.4 mM EDTA

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Previous videos:

Genetically modify Bacteria /youtube/video/F0_q-fD_lyU

Building an Incubator /youtube/video/Zm378x6zJYc

Spider Beer 1 /youtube/video/Fx8TcGrCOSI

DNA Extraction /youtube/video/knosqmvLWSc

Growth Media /youtube/video/L0o51eco6Vg

PCR /youtube/video/7kJ2o7P8D00

Fluorescent Microscope /youtube/video/iYoAiqufB7w

Gel electrophoresis /youtube/video/sSKls2kNC4U

How DNA was Discovered /youtube/video/4pRYHrPJ8Eo

How to stock a genetics lab /youtube/video/zqQ4x9rmkKg

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Intro
/youtube/video/DHNPnO5UOYQ?t=0
What is betacarotene
/youtube/video/DHNPnO5UOYQ?t=30
How does this work
/youtube/video/DHNPnO5UOYQ?t=168
Lycopene
/youtube/video/DHNPnO5UOYQ?t=265
Genes
/youtube/video/DHNPnO5UOYQ?t=340
Yeast
/youtube/video/DHNPnO5UOYQ?t=490
Tools
/youtube/video/DHNPnO5UOYQ?t=528
Preparation
/youtube/video/DHNPnO5UOYQ?t=613
Yeast strain
/youtube/video/DHNPnO5UOYQ?t=650
Yeast preparation
/youtube/video/DHNPnO5UOYQ?t=673
Washing the yeast
/youtube/video/DHNPnO5UOYQ?t=699
Heat shocking
/youtube/video/DHNPnO5UOYQ?t=732
Incubation
/youtube/video/DHNPnO5UOYQ?t=765
Frederick
/youtube/video/DHNPnO5UOYQ?t=813
Phyto into lycopene
/youtube/video/DHNPnO5UOYQ?t=864
Sugar Wine
/youtube/video/DHNPnO5UOYQ?t=911
Sourdough Bread
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Express VPN
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Patreon patreon.com
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