2019-02-01

[unlisted] 158K views, 6.15K likes, 124 dislikes audio only

The Thought Emporium

Bioreactors are very simple, but extremely useful devices. They're basically a large tank that microorganisms can be grown in, but what makes them special is their ability to grow a nearly infinite amount of the organism or a product an organism produces by slowly feeding in fresh nutrients and pulling out small amounts of product. They can also be used for directed evolution experiments, and so much more.

I wanted one for all of these reasons and more, but also so that I had a nice visual to talk about the many bioluminescent microbes I've collected. So in this video we go through the process of turning a kerosene storm lantern into a bioreactor. In a future video we'll build a pumping and heating unit to accompany the build to make this into a fully capable bioreactor.

For the first test I grew some E. coli that produce red fluorescent protein and after only a few days of growth the color was clear to see.

David Ishee's channel: https://www.youtube.com/channel/UCftNyK6c9JdIFTQ2TLQqm_g

Spider Silk Playlist: /youtube/video/Fx8TcGrCOSI

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