video thumbnail 15:41
Building a Nanodrop Style UV/Vis Spectrometer

2019-01-18

[public] 166K views, 8.86K likes, 71.0 dislikes audio only

channel thumbThe Thought Emporium

Spectrometers are one of the most ubiquitous tools in most labs because an enormous amount of information about a substance can be gleaned from how it interacts with various parts of the electromagnetic spectrum. But for as common as they are, the price tag on a new spectrometer can be massive.

Today we go through a bit of theory on the electromagnetic spectrum so we understand spectrometery, and then build a little nanodrop-esq spectrometer for use in the lab. While it's still a work in progress, it's more than sufficient to preform many basic tests and experiments.

Applied science Zeeman effect: /youtube/video/OzkcB1lkgGU

Hackteria: https://hackteria.org/wiki/DIY_NanoDrop

SVG file of design: https://github.com/FOULAB/DIYnanodrop/blob/master/DIYNanoDrop.svg

Webcam: https://www.amazon.ca/AUSDOM-1920x1080P-Noise-cancelling-Recording-Computer/dp/B017SRHRR6/ref=sr_1_7?crid=8KPDXFH5IJ93&keywords=hd+webcam&qid=1547857754&sprefix=hd+web%2Caps%2C175&sr=8-7

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splitting the normally mixed white light into all the various colors
/youtube/video/pIk8I10ZmYY?t=59.049999
measure that light with a spectrometer
/youtube/video/pIk8I10ZmYY?t=85.779999
jumping points
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build a spectrometer
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gave all the wooden pieces a quick paint job
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pipe two different light sources through the spectrometer
/youtube/video/pIk8I10ZmYY?t=326.56
gluing it back into the main plate
/youtube/video/pIk8I10ZmYY?t=355.02899
mount the piece of mirror onto the mirror mounting plate
/youtube/video/pIk8I10ZmYY?t=404.57001
hold the mirror flat onto the wood
/youtube/video/pIk8I10ZmYY?t=410.42999
cut a small square in the bandsaw
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feed the camera wire through the spot on the back
/youtube/video/pIk8I10ZmYY?t=442.47
used some aluminium tape on the underside
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turn on the white led on top
/youtube/video/pIk8I10ZmYY?t=559.57001
use the power supply for the camera
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plug any remaining holes
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calibrate the software
/youtube/video/pIk8I10ZmYY?t=652.03003
keep the light source constant rather than looking at different light sources
/youtube/video/pIk8I10ZmYY?t=688.17999
place each in the path of the light and measure
/youtube/video/pIk8I10ZmYY?t=706.95898
a calibration curve
/youtube/video/pIk8I10ZmYY?t=718.14899
use a mixture of antibodies
/youtube/video/pIk8I10ZmYY?t=737.04999
measure the absorbance of the solution at about 600 nanometers
/youtube/video/pIk8I10ZmYY?t=755.16998
see a sharp peak from the dyeing the plastic emitting photons
/youtube/video/pIk8I10ZmYY?t=800.28998
start to fluoresce under uv light by measuring how much light
/youtube/video/pIk8I10ZmYY?t=821.14001
shift spectral lines using powerful magnets
/youtube/video/pIk8I10ZmYY?t=912.37