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Using DIY CRYOGEL to Keep Mammalian Cells Alive at Room Temp

2018-02-18

[unlisted] 26.8K views, 1.13K likes, 7.00 dislikes audio only

Storage and transport is a vital part of any lab that does cell culture, but for anything other than bacteria and fungi, the ways to do this require extreme cold. Here we demonstrate how to prepare a ultralight foam from cross linked gelatin, which can be used to keep cells alive for up to a week at room temperature. No incubator, CO2, dry ice or liquid nitrogen required. The material is easy to make and prepare and the method works for both adherent and suspension cells.

This was filmed while my friend was letting me do the lactose project in his lab. Since filming, he's used this method to ship cells and they survived and were able to be cultured.

Link to original paper: https://www.nature.com/articles/srep41551

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The Thought Emporium Science is a bottomless well of some of the most amazing things this universe has to offer. There is so much amazing science and engineering that gets done, but most often the only ones who get to experience it first hand are those in expensive labs in universities. This channel aims to explore as much of fields of science and engineering as possible by showing real experiments and research being done in a fun and accessible way. While the goal is to show that even very hard science can be done in a DIY way, some projects will use some of the fancier toys that while less accessible, opens a broader range of projects that can be done. We cover everything from genetic engineering and tissue culture, to nuclear physics, to machine engineering, to chemical synthesis and more.
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